Nucleotídeos na Associação de Microtúbulos
Properties of tubulin-assembling nucleotides
Nucleotide analog | Properties |
---|---|
ITP | highly promotive |
GTP | standard for reactivity of other analogs |
GpCpp (GMPCPP) | moderately promotive, completely suppresses dynamic instability - microtubules do not depolymerize |
caged-GpCpp (caged-GMPCPP) | "caged" derivative of GpCpp (GMPCPP) - blocked by a photo-labile group |
6-Methylthio-GTP | moderately promotive |
8Br-GTP | moderately promotive |
dGTP | moderately promotive |
mant-GTP | slightly inhibitory |
XTP | slightly inhibitory |
6-Thio-GTP | highly inhibitory |
Produtos
Selected References
Läppchen et al. (2005) GTP Analogue Inhibits Polymerization and GTPase Activity of the Bacterial Protein FtsZ without Affecting Its Eukaryotic Homologue Tubulin. Biochemistry 44 (21):7879.
Muraoka et al. (1999) Effects of Purinenucleotide Analogues on Microtubule Assembly. Cell Structure and Function 24:305.
Hyman et al. (1992) Role of GTP Hydrolysis in Microtubule Dynamics: Information from a Slowly Hydrolyzable Analogue, GMPCPP. Mol. Biol. of the Cell 3:1155, and references therein.