Nucleotídeos Fluorescentes para Marcação de DNA
Table 1: Enzymatic incorporation of fluorescent Nucleotides.
PCR: Polymerase chain reaction with Taq Polymerase; MDA-based WGA: Multiple displacement amplification (MDA)-based whole genome amplification (WGA) with Phi29 DNA Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)
DNA Labeling | cDNA Labeling | |||||||
---|---|---|---|---|---|---|---|---|
Emission color | Nucleotide | Cat. No. | PCR | NT | Primer Extension | 3' End Labeling | RT | |
dUTP | ||||||||
blue | AMCA-dUTP | NU-803-AMCA | - | ✔2 | ✔2 | n/a | n/a | n/a |
DEAC-dUTP | NU-803-DEAC | - | ✔1 | n/a | n/a | n/a | ||
dUTP-ATTO-425 | NU-803-425 | ✔1 | ✔1,2 | ✔1 | ✔1 | n/a | ||
dUTP-XX-ATTO-425 | NU-803-XX-425 | ✔1 | ✔1 | n/a | n/a | n/a | ||
green | dUTP-XX-AF488 | NU-803-XX-AF488 | ✔1 | ✔1 | n/a | n/a | n/a | |
dUTP-XX-ATTO-488 | NU-803-XX-488 | ✔1,2 | ✔1,2 | ✔1,2 | ✔1,2 | n/a | ||
Fluorescein-12-dUTP | NU-803-FAMX | ✔1,2 | ✔1,2 | ✔1,2 | ✔1,2 | n/a | ||
Rhodamine-12-dUTP | NU-803-RHOX | - | ✔1,2 | ✔1 | ✔1,2 | n/a | ||
yellow- green | dUTP-XX-ATTO-532 | NU-803-532 | ✔1 | ✔1 | ✔1 | ✔1 | n/a | |
yellow | dUTP-Cy3 | NU-803-Cy3 | ✔1,2 | ✔1,2 | ✔1 | ✔1 | ✔2 | |
dUTP-XX-Cy3 | NU-803-XX-CY3 | ✔1 | ✔1 | ✔1 | n/a | ✔1 | ||
dUTP-XX-AF555 | NU-803-XX-AF555 | ✔1 | ✔1 | n/a | n/a | n/a | ||
dUTP-ATTO-550 | NU-803-550 | - | ✔1 | ✔1 | ✔1 | n/a | ||
orange | dUTP-Texas Red | NU-803-TXR | ✔1,2 | ✔1,2 | ✔2 | n/a | n/a | |
dUTP-XX-Texas Red | NU-803-XX-TXR | ✔1,2 | ✔1,2 | ✔2 | n/a | n/a | ||
dUTP-XX-AF594 | NU-803-XX-AF594 | ✔1,2 | ✔1,2 | ✔1 | n/a | n/a | ||
dUTP-XX-594 | NU-803-XX-594 | ✔1 | ✔1 | ✔1 | ||||
red | dUTP-Cy5 | NU-803-CY5 | ✔1,2 | ✔1,2 | ✔1 | n/a | ✔2 | |
dUTP-XX-Cy5 | NU-803-XX-CY5 | ✔1 | ✔1 | ✔1 | n/a | ✔1 | ||
dUTP-ATTO-647N | NU-803-647N | - | ✔1,2 | ✔1 | ✔1 | n/a | ||
dUTP-ATTO-643 | NU-803-643 | ✔1 | ✔1 | ✔1 | ||||
dUTP-XX-ATTO-643 | NU-803-XX-643 | ✔1 | ✔1 | ✔1 | ||||
dUTP-XX-AF647 | NU-803-XX-AF647 | ✔1 | ✔1 | n/a | n/a | n/a | ||
dUTP-XX-STAR RED | NU-803-XX-STRED | ✔1 | n/a | n/a | n/a | n/a | ||
dUTP-ATTO-655 | NU-803-655 | - | ✔1 | ✔1 | ✔1 | n/a | ||
IR-red | dUTP-XX-IR680LT | NU-803-XX-IR680LT | ✔1 | n/a | n/a | n/a | n/a | |
dUTP-XX-IR750 | NU-803-XX-IR750 | ✔1 | n/a | n/a | n/a | n/a | ||
dCTP | ||||||||
green | Fluorescein-12-dCTP | NU-809-FAMX | ✔1 | ✔1 | n/a | n/a | n/a | |
Rhodamine-12-dCTP | NU-809-RHOX | - | ✔1 | n/a | n/a | n/a | ||
yellow | dCTP-Cy3 | NU-809-Cy3 | ✔1 | ✔1 | n/a | n/a | n/a | |
dCTP-ATTO-550 | NU-809-550 | - | ✔1 | n/a | n/a | n/a | ||
orange | dCTP-Texas Red | NU-809-TXR | ✔1 | ✔1 | n/a | n/a | n/a | |
red | dCTP-Cy5 | NU-809-CY5 | ✔1 | ✔1 | n/a | n/a | n/a | |
dCTP-ATTO-647N | NU-809-647N | - | ✔1 | n/a | n/a | n/a |
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable