5-Ethynyl-UTP (5-EUTP)
5-Ethynyl-uridine-5'-triphosphate, Sodium salt
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
CLK-T08-S | 5 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
CLK-T08-L | 5 x 5 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
CLK-T08-XL | 50 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C11H15N2O15P3 (free acid)
Peso molecular: 508.16 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 100 mM - 110 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription.
The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
- to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)
- to crosslink the RNA to azide-functionalized biomolecules e.g.proteins
Produtos relacionados:
- Copper (II)-Sulphate (CuSO4), #CLK-MI004
- Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
- Sodium Ascorbate (Na-Ascorbate), #CLK-MI005
Referências selecionadas:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.