5-Propargylamino-dCTP - Solution
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
NU-809S | 10 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
NU-809L | 5 x 10 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
NU-809XL | 100 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C12H19N4O13P3 (free acid)
Peso molecular: 520.22 g/mol (free acid)
CAS#: 115899-39-3
Pureza: ≥ 93 % (HPLC)
Forma: solution in water
Concentração: 100 mM - 110 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into DNA by
- PCR with Taq polymerasein-house data
Descrição:
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasksto introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging