UDP-6-azide-glucose
Sodium salt
UDP-6-N3-Glc, 6-N3-UDPG
Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
---|---|---|---|
CLK-076 | 0,5 mg | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C15H23N5O16P2
Peso molecular: 591.32 g/mol
Pureza: ≥ 98 % (HPLC)
Forma: solid
Solubilidade: water, aqueous buffer such as PBS
Formulários:
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ß-Glycosyltransferase (T4 ß-GT)[1-3]
The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice
- to introduce a (Desthio)Biotin group for subsequent purification tasks (via DBCO-containing (Desthio)Biotin)
- to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes)
Referências selecionadas:
[1] Song et al. (2011) Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nature Biotech 29(1):68.
[2] Li et al. (2012) Selective Capture of 5-hydroxymethylcytosine from Genomic DNA. J. Vis. Exp. 68:e4441.
[3] Song et al. (2016) Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation. PNAS 113(16):4339.